This is the PDF eBook version for Synthetic mRNA – Production, Introduction Into Cells, and Physiological Consequences by Robert E. Rhoads

Part I Introduction

1. Synthetic mRNA: Production,
Introduction into Cells, and Physiological Consequences
Robert E. Rhoads

Part
II Synthesis of mRNA

2.
Synthetic
Capped mRNAs for Cap-Specific Photo-Crosslinking Experiments
Joanna Kowalska, Franck Martin, and Jacek Jemielity

3. Enzymatic Modification of 5’-Capped
RNA and Subsequent Labeling by Click Chemistry
Josephin M. Holstein, Daniela Stummer, and
Andrea Rentmeister

4. Preparation of Functional,
Fluorescently Labeled mRNA Capped with Anthraniloyl-m7GpppG
Artem V. Domashevskiy, David J.
Rodriguez, Dilantha Gunawardana, and Dixie J. Goss

5. Intronless b-Globin
Reporter: A Tool for Studying Nuclear
RNA Stability Elements
Jessica A. Brown and Joan A. Steitz

6.
Synthetic mRNA with Superior Properties that Mimics the Intracellular Fates of Natural Histone mRNA
Wei Su, Michael K. Slevin, William F. Marzluff, and Robert E.
Rhoads

7. Engineering WT1-Encoding mRNA to
Increase Translational Efficiency in Dendritic Cells
Daphné Benteyn, Carlo Heirman, Kris
Thielemans, and Aude Bonehill

Part
III Introduction of Synthetic mRNA into Cells

8. Electroporation of Alphavirus RNA
Translational Reporters into Fibroblastic and Myeloid Cells as a Tool to Study the Innate Immune System
Christina L. Gardner, Derek W. Trobaugh,
Kate D. Ryman, and William B. Klimstra

9. GMP-Grade mRNA Electroporation of
Dendritic Cells for Clinical Use
Judith Derdelinckx, Zwi N. Berneman, and Nathalie
Cools

10. Large Scale mRNA Transfection of
Dendritic Cells by Electroporation in Continuous Flow Systems
David Selmeczi, Thomas Steen Hansen,
Özcan Met, Inge Marie Svane, and Niels B. Larsen

11. FLT3 Ligand as a Molecular Adjuvant for Naked RNA Vaccines
Sebastian Kreiter, Mustafa Diken,
Abderraouf Selmi, Jutta Petschenka, Özlem Türeci, and Ugur Sahin

12. Transfecting Human Monocytes with
RNA
Jens Dannull and Smita K. Nair

13. In Vitro Synthesis, Delivery and
Bioavailability of Exogenous mRNA in Gene Transfer Mediated by PiggyBac Transposition
Solenne Bire, Nicole Ishac, and Florence
Rouleux-Bonnin

14. Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage
Gábor Boros, Katalin Karikó, Hiromi
Muramatsu, Edit Miko, Eszter Emri, Csaba Hegedus, Gabriella Emri, and Éva
Remenyik

Part
IV Alteration of Cellular Function with Synthetic mRNA

15. Delivery of Synthetic mRNA Encoding
FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy
Gayathri R. Devi and Sritama Nath
16. Immune Monitoring using mRNA-Transfected
Dendritic Cells
Troels Holz Borch, Inge Marie Svane, and Özcan
Met

17. Transfection of Tumor-Infiltrating
T Cells with mRNA Encoding CXCR2
Manja Idorn, Per thor Straten, Inge Marie
Svane, and Özcan Met

18. mRNA Electroporation of Dendritic
Cells with WT1, Survivin and TriMix (A Mixture of caTLR4, CD40L, and CD70)
An Coosemans, Sandra Tuyaerts, Kim
Morias, Jurgen Corthals, Carlo Heirman, Kris Thielemans, Stefaan W. Van Gool,
Ignace Vergote, and Frédéric Amant

19. Redirecting T Cell Specificity
Using T Cell Receptor Messenger RNA Electroporation
Sarene Koh, Noriko Shimasaki, and Antonio
Bertoletti

20. Measuring Hematocrit in Mice Injected with In Vitro-Transcribed Erythropoietin mRNA
Azita Josefine Mahiny and Katalin Karikó

21. Genetic Modification of Human
Pancreatic Progenitor Cells through Modified mRNA
Song Lu, Christie C. Chow, Junwei Zhou,
Po Sing Leung, Stephen K. Tsui, and Kathy O. Lui