(PDF) Malaria Vaccines – Methods and Protocols by Ashley Vaughan

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Table of Contents

Part I Pre-Erythrocytic Stages

1. Isolation of Non-Parenchymal Cells from the Mouse Liver
Isaac Mohar, Katherine J. Brempelis, Sara A. Murray, Mohammad R. Ebrahimkhani, and I. Nicholas Crispe

2. Measurement of the T Cell Response to Pre-Erythrocytic Vaccination in Mice
Jenna J. Guthmiller, Ryan A. Zander, and Noah S. Butler

3. Characterization of Liver CD8 T cell Subsets that are Associated with Protection against Pre-Erythrocytic Plasmodium Parasites
Stasya Zarling and Urszula Krzych

4. Flow Cytometry-Based Assessment of Antibody Function against Malaria Pre-Erythrocytic Infection
Alyse N. Douglass, Peter G. Metzger, Stefan H.I. Kappe, and Alexis Kaushansky

5. Assessment of Parasite Liver Stage Burden in Human-Liver Chimeric Mice
Lander Foquet, Philip Meuleman, Rob Hermsen, Robert Sauerwein, and Geert Leroux-Roels

6. Measurement of Antibody-Mediated Reduction of Plasmodium Yoelii Liver Burden by Bioluminescent Imaging
Brandon K. Sack, Jessica L. Miller, Ashley M. Vaughan and Stefan H. I. Kappe

7. Detection of Plasmodium Berghei and Plasmodium Yoelii Liver-Stage Parasite Burden by Quantitative Real Time PCR
Alexander Pichugin and Urszula Krzych

Part II Mosquito Stages

8. Membrane Feeding Assay to Determine the Infectiousness of Plasmodium Vivax Gameytes
Jetsumon Sattabongkot Prachumsri, Chalermpon Kumpitak, and Kirakorn Kiattibutr

9. The Standard

Membrane Feeding Assay: Advances Using
Bioluminescence
Will J. R. Stone and Teun Bousema
Part III Erythrocytic Stages

10. Agglutination Assays of the Plasmodium Falciparum Infected Erythrocyte
Joshua Tan and Peter C. Bull

11. Antibody-Dependent Cell-Mediated Inhibition (ADCI) of Plasmodium Falciparum: One and Two-Step ADCI Assays
Hasnaa Bouharoun-Tayoun and Pierre Druilhe

12. A Robust Phagocytosis Assay to Evaluate the Opsonic Activity of Antibodies against Plasmodium Falciparum-Infected Erythrocytes
Andrew Teo, Wina Hasang, Philippe Boeuf, and Stephen Rogerson

13. Miniaturized Growth Inhibition Assay to Assess the Anti-Blood Stage Activity of Antibodies
Elizabeth H. Duncan and Elke S. Bergmann-Leitner

14. Measuring Plasmodium Falciparum Erythrocyte Invasion Phenotypes Using Flow Cytometry
Amy Kristine Bei and Manoj T. Duraisingh

15. The In Vitro Invasion Inhibition Assay (IIA) for Plasmodium Vivax
Wanlapa Roobsoong

16. The Ex Vivo IFN-? Enzyme-Linked Immunospot (ELISpot) Assay
Martha Sedegah

17. Evaluating IgG Antibody to Variant Surface Antigens Expressed on Plasmodium Falciparum Infected Erythrocytes Using Flow Cytometry
Andrew Teo, Wina Hasang and Stephen Rogerson

18. Inhibition of Infected Red Blood Cell Binding to the Vascular Endothelium

Marion Avril
19. Evaluation of Pregnancy Ma

laria V
accine
Candidates: The Binding Inhibition Assay
Tracy Saveria, Patrick E. Duffy, and Michal Fried
20. High-Throughput Testing of Antibody Dependent Binding Inhibition of Placental Malaria Parasites
Morten A. Nielsen and Ali Salanti

Part IV Parasite Manipulation

21. Generation of Transgenic Rodent Malaria Parasites Expressing Human-Malaria Parasite Proteins
Ahmed M. Salman, Catherin Marin Mogollon, Jing-wen Lin, Fiona J.A. van Pul, Chris J. Janse, and Shahid M. Khan

Part V Vaccination

22. Vaccination Using Gene-Gun Technology
Elke Bergmann-Leitner and Wolfgang W. Leitner