(PDF) G Protein-Coupled Receptors in Drug Discovery 2nd Edition by Marta Filizola

This is the PDF eBook version for G Protein-Coupled Receptors in Drug Discovery – Methods and Protocols 2nd Edition by Marta Filizola

Table of Contents

1. Purification of Stabilized GPCRs for Structural and Biophysical Analyses

James C. Errey, Andrew S. Doré, Andrei Zhukov, Fiona H. Marshall, and Robert M. Cooke

2. Purification and Crystallization of a Thermostabilized Agonist-Bound Conformation of the Human Adenosine A2A Receptor

Christopher G. Tate and Guillaume Lebon

3. 2D Projection Analysis of GPCR Complexes by Negative Stain Electron Microscopy

Alys Peisley and Georgios Skiniotis

4. Nuts and Bolts of CF3 and CH3 NMR Towards the Understanding of Conformational Exchange of GPCRs

R. Scott Prosser and Tae Hun Kim

5. Single-Molecule Fluorescence Microscopy for the Analysis of Fast Receptor Dynamics

Julia Wagner, Titiwat Sungkaworn, Katrin G. Heinze, Martin J. Lohse, and Davide Calebiro

6. Quantitative Multi-Color Detection Strategies for Bioorthogonally-Labeled GPCRs

Minyoung Park, He Tian, Saranga Naganathan, Thomas P. Sakmar, and Thomas Huber

7. Approaches to Characterize and Quantify Oligomerization of GPCRs

Sara Marsango, María José Varela Liste, and Graeme Milligan

8. Monitoring G Protein Activation in Cells with BRET

Ikuo Masuho, Kirill A. Martemyanov, and Nevin A. Lambert

9. Use of Fluorescence Indicators in Receptor Ligands

Kaleeckal G. Harikumar and Laurence J. Miller

10. Detection and Quantification of Intracellular Signalling Using FRET-Based Biosensors and High Content Imaging

Michelle L. Halls, Daniel P. Poole, Andrew M. Ellisdon, Cameron J. Nowell, and Meritxell Canals

11. The Measurement of Receptor Signaling

Bias
Terry Kenakin

12. Approaches to Assess Functional Selectivity in GPCRs: Evaluating G Protein Signaling in an Endogenous Environment

Laura M. Bohn, Lei Zhou, and Jo-Hao Ho

13. Bioluminescence Resonance Energy Transfer Approaches to Discover Bias in GPCR Signaling

Elizabeth K.M. Johnstone and Kevin D.G. Pfleger

14. Virus-Mediated Expression of DREADDs for In Vivo Metabolic Studies

Mario Rossi, Zhenzhong Cui, Ken-ichiro Nakajima, Jianxin Hu, Lu Zhu, and Jürgen Wess

15. High-Throughput Screening for Allosteric Modulators of GPCRs

Robert L. Bertekap, Jr., Neil T. Burford, Zhuyin Li, and Andrew Alt

16. Radioligand Binding Assay for an Exon 11-Associated Mu Opioid Receptor Target

Gina F. Marrone, Susruta Majumdar, and Gavril W. Pasternak

17. Docking and Virtual Screening Strategies for GPCR Drug Discovery

Thijs Beuming, Bart Lenselink, Daniele Pala, Fiona McRobb, Matt Repasky, and Woody Sherman

18. The Dynamic Process of Drug-GPCR Binding at either Orthosteric or Allosteric Sites Evaluated by Metadynamics

Sebastian Schneider, Davide Provasi, and Marta Filizola

19. Experiment-Guided Molecular Modeling of Protein-Protein Complexes Involving GPCRs

Irina Kufareva, Tracy M. Handel, and Ruben Abagyan

20. Interaction Fingerprints and Their Applications to Identify Hot Spots

Andre’s F. Marmolejo, José L. Medina-Franco, Marc Giulianotti, and Karina Martinez-Mayorga